禽波氏菌强毒株感染雏鸡呼吸系统荷菌量及病变规律的研究

doi:10.11843/j.issn.0366-6964.2017.04.013

摘要/Abstract

摘要：

禽波氏菌为产碱杆菌科波氏杆菌属成员，主要导致咳嗽等呼吸道症状。为探索禽波氏菌经呼吸道的入侵过程及其病变发生发展规律，本试验利用筛选到的强毒株经鼻腔感染SPF雏鸡，在感染后的不同时间检测气管和肺荷菌量的变化，通过扫描电镜观察禽波氏菌在气管的定植及其病变规律，利用间接免疫酶组化研究禽波氏菌在肺中的定植规律，分析肺和支气管的病理组织学变化。结果发现，在感染之初气管中细菌数量较多，而肺中较少，之后气管和肺的荷菌量均呈明显上升趋势。感染后1 d即发现禽波氏菌已经在气管纤毛上定植，且纤毛开始出现脱落；到5 d时气管黏膜表面呈斑驳状，纤毛大面积脱落，甚至出现空洞。肺在感染后2 d检测出细菌定植，主要分布在肺房和呼吸毛细管壁的细胞质中，之后随着感染时间的延长，阳性信号不断增加。肺和支气管的病理变化在5 d时出现，主要表现淤血；至感染后10 d，大量细胞坏死。试验结果表明所筛选禽波氏菌分离株对雏鸡的呼吸道具有很强的亲嗜性，优先定植于气管上皮细胞的纤毛上，并随之在气管、支气管和肺产生一系列的组织病理学变化。

Abstract:

Bordetella avium (B. avium), one of the members of Bordetella genus, Alcaligenes Branch, mainly causes birds cough and other respiratory symptoms. To explore its invasive procedure by respiratory tract and regular pattern of lesions development, SPF chicks were challenged by nasal infection with a screened virulent B. avium strain. After infection, bacteria quantity in trachea and lungs were detected at different times. Tracheas colonization and lesions were observed by SEM. Lungs colonization pattern were detected by indirect immunoenzymatic staining. Histopathological changes in lung and bronchus were detected by pathological examination. It was found that bacterial infection of the trachea was in larger quantities, and only a small amount in the lungs at the beginning. And then the amount of bacteria in trachea and lungs showed a clear upward trend. B. avium had colonized on the cilia of trachea, and cilia began to fall off on 1 d after infection. To 5 d after infection, tracheal mucosal surface mottled, cilia fell off in a large area, and even holes appeared. Lungs colonization were detected on 2 d after infection, and B. avium were mainly distributed in the cytoplasm of lung housing and respiratory capillary walls. Positive signals were increasing with the extension of time. Pathological changes in the lungs and bronchi appeared on 5 d after infection, mainly showed congestion. To 10 d after infection, a large number of cell necrosis appeared. In conclusion, this B. avium isolate has a strong tropism on chick respiratory tract. Especially it has priority colonization on cilia of tracheal epithelial cells, and subsequently causes a series of histopathological changes in the trachea, bronchi and lungs.

中图分类号:

S852.61

杨萍萍, 袁朋, 魏凯, 胡莉萍, 朱瑞良. 禽波氏菌强毒株感染雏鸡呼吸系统荷菌量及病变规律的研究[J]. 畜牧兽医学报, 2017, 48(4): 699-705.

YANG Ping-ping, YUAN Peng, WEI Kai, HU Li-ping, ZHU Rui-liang. Study on Colonization and Pathological Changes of Chick Respiratory Tract Induced by Bordetella avium Virulent Isolate[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 699-705.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2017.04.013

https://www.xmsyxb.com/CN/Y2017/V48/I4/699

参考文献

[1] NOVIKOV A, SHAH N R, ALBITAR-NEHME S, et al. Complete Bordetella avium, Bordetella hinzii and Bordetella trematum lipid A structures and genomic sequence analyses of the loci involved in their modifications[J]. Innate Immun, 2014, 20(6):659-672.
[2] 李洪广, 王芳, 姜平, 等. 兔支气管败血波氏杆菌PRN基因缺失突变株的构建及特性研究[J]. 畜牧兽医学报, 2012, 43(2):299-305.
LI H G, WANG F, JIANG P, et al. Construction of defined mutations of PRN of rabbit Bordetella bronchiseptica and property research[J]. Acta Veterinaria et Zootechnica Sinica, 2012, 43(2):299-305. (in Chinese)
[3] SRIGLEY J A,GOLDFARB D M,PERNICA J M. Bordetella species other than Bordetella pertussis[J]. Clin Microbiol Newslett, 2015, 37(8):61-65.
[4] KAMACHI K, YOSHINO S, KATSUKAWA C, et al. Laboratory-based surveillance of pertussis using multitarget real-time PCR in Japan:evidence for Bordetella pertussis infection in preteens and teens[J]. New Microbes New Infect, 2015, 8:70-74.
[5] WANG C W, LIU L P, ZHANG Z, et al. Immunological and protective effects of Bordetella bronchiseptica subunit vaccines based on the recombinant N-terminal domain of dermonecrotic toxin[J]. Int Immunopharmacol, 2015, 28(2):952-959.
[6] IVANOV Y V, SHARIAT N, REGISTER K B, et al. A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease[J]. BMC Genomics, 2015, 16:863.
[7] SEBAIHIA M, PRESTON A, MASKELL D J, et al. Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction[J]. J Bacteriol, 2006, 188(16):6002-6015.
[8] BEACH N M, THOMPSON S, MUTNICK R, et al. Bordetella avium antibiotic resistance, novel enrichment culture, and antigenic characterization[J]. Vet Microbiol, 2012, 160(1-2):189-196.
[9] LOKER S B, TEMPLE L M, PRESTON A. The Bordetella avium BAV1965-1962 fimbrial locus is regulated by temperature and produces fimbriae involved in adherence to turkey tracheal tissue[J]. Infect Immun, 2011, 79(6):2423-2429.
[10] SUN Z H, WEI K, YAN Z G, et al. Effect of immunological enhancement of aloe polysaccharide on chickens immunized with Bordetella avium inactivated vaccine[J]. Carbohydr Polym, 2011, 86(2):684-690.
[11] LIU L P, YU C L, WANG C W, et al. Immuno-enhancement of Taishan Pinus massoniana pollen polysaccharides on recombinant Bordetella avium ompA expressed in Pichia pastoris[J]. Microb Pathog, 2016, 95:54-61.
[12] ZHU F J, LIU X, SUN Z H, et al. Immune-enhancing effects of Taishan pinus massoniana pollen polysaccharides on DNA vaccine expressing Bordetella avium ompA[J]. Front Microbiol, 2016, 7:66.
[13] ZHAO X, LIANG M F, YANG P P, et al. Taishan Pinus massoniana pollen polysaccharides promote immune responses of recombinant Bordetella avium ompA in BALB/c mice[J]. Int Immunopharmacol, 2013, 17(3):793-798.
[14] 谭燕玲, 朱瑞良, 王慧, 等. 鸡胚胎性病原菌多重PCR检测方法的建立[J]. 中国预防兽医学报, 2011, 33(5):374-377.
TAN Y L, ZHU R L, WANG H, et al. Establishment of multiplex PCR detection for pathogens of chicken embryos[J]. Chinese Journal of Preventive Veterinary Medicine, 2011, 33(5):374-377. (in Chinese)
[15] LIANG M F, ZHAO Q Y, LIU G H, et al. Pathogenicity of Bordetella avium under immunosuppression induced by Reticuloendotheliosis virus in specific-pathogen-free chickens[J]. Microb Pathog, 2013, 54:40-45.
[16] GUO F X, XUE C, WU C, et al. Effects of polysaccharide on chicks co-infected with Bordetella avium and Avian leukosis virus[J]. Carbohydr Polym, 2014, 109:71-76.
[17] MIYAMOTO D M, RUFF K, BEACH N M, et al. Bordetella avium causes induction of apoptosis and nitric oxide synthase in turkey tracheal explant cultures[J]. Microbes Infect, 2011, 13(10):871-879.
[18] MORENO L Z, KNÖBL T, GRESPAN A A, et al. Draft genome sequence of Bordetella avium Nh1210, an outbreak strain of lockjaw syndrome[J]. Genome Announc, 2015, 3(2):e00120-15.
[19] RAFFEL T R, REGISTER K B, MARKS S A, et al. Prevalence of Bordetella avium infection in selected wild and domesticated birds in the eastern USA[J]. J Wildl Dis, 2002, 38(1):40-46.
[20] SPILKER T, LIWIENSKI A A, LIPUMA J J. Identification of Bordetella spp. in respiratory specimens from individuals with cystic fibrosis[J]. Clin Microbiol Infect, 2008, 14(5):504-506.
[21] HARRINGTON A T, CASTELLANOS J A, ZIEDALSKI T M, et al. Isolation of Bordetella avium and novel Bordetella strain from patients with respiratory disease[J]. Emerg Infect Dis, 2009, 15(1):72-74.
[22] 覃娟娟, 顾凡, 蔡雨涵, 等. 副猪嗜血杆菌毒力菌株和猪支气管败血波氏杆菌毒力菌株双重PCR检测方法的建立及应用[J]. 中国兽医学报, 2015, 35(8):1217-1222.
QIN J J, GU F, CAI Y H, et al. Establishment and application of duplex PCR for detecting virulent strains of Haemophilus parasuis and Bordetella bronchiseptica[J]. Chinese Journal of Veterinary Science, 2015, 35(8):1217-1222. (in Chinese)
[23] HITTLE L E, JONES J W, HAJJAR A M, et al. Bordetella parapertussis PagP mediates the addition of two palmitates to the lipopolysaccharide lipid A[J]. J Bacteriol, 2015, 197(3):572-580.
[24] MAESHIMA N, EVANS-ATKINSON T, HAJJAR A M, et al. Bordetella pertussis lipid A recognition by toll-like receptor 4 and MD-2 is dependent on distinct charged and uncharged interfaces[J]. J Biol Chem, 2015, 290(21):13440-13453.
[25] 胡思顺, 王晓丽, 陆时娟, 等. 鸡毒支原体HS株pMGA1.2重组蛋白的免疫保护性研究[J]. 中国预防兽医学报, 2015, 37(3):225-228.
HU S S, WANG X L, LU S J, et al. The immunity of Mycoplasma gallisepticum strain HS recombinant adhesion protein rpMGA1.2[J]. Chinese Journal of Preventive Veterinary Medicine, 2015, 37(3):225-228. (in Chinese)
[26] 杨萍萍, 刘静静, 赵雪, 等. 间接免疫酶组化检测禽波氏杆菌在感染鸡体内的抗原定位[J]. 中国兽医学报, 2013, 33(12):1832-1837.
YANG P P, LIU J J, ZHAO X, et al. Study on localization of Bordetella avium in infected chickens by indirect immunoperoxidase techinique[J]. Chinese Journal of Veterinary Science, 2013, 33(12):1832-1837. (in Chinese)
[27] TEMPLE L M, MIYAMOTO D M, MEHTA M, et al. Identification and characterization of two Bordetella avium gene products required for hemagglutination[J]. Infect Immun, 2010, 78(6):2370-2376.